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KMID : 0352819930090010031
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Kosin Medical Journal
1993 Volume.9 No. 1 p.31 ~ p.40
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Effect of cadmium Intoxication on Organic Cation Transport System in Proximal Tubular Luminal Membrance
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Abstract
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Effect of cadmium intoxication on the organic transport system in renal cortical brush-boredr membranes was studied in adult male Sprague-Dawley rats. Cadimium intoxication was induced by subcutaneous injections of CdCl2 at a dose of 2 mg Cd/kg
body
weight per day for 3 weeks. Renalplasma membrane vesicles were isolated by Percoll gradient centrifugation and magnesium precipitation, and the substrate uptake by vesicles was determined by a rapid filtration technique using 14C-labeled
tetraethylammonium(TER).
In renal cortical brush-border membrane vesicles of cadmium-intoxicated rats, the H+ _TEA exchange was warkedly affenuated, and this was due to a reduction in Vmax with no change in Km.
These results suggest that cadmium intoxication impairs the H+ -organec cation exchange system in the proximal tubular brush-border membranes by reducing the effective number of carriers, and this may lead to defcct in organic cation excretion in
intact
animals.
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